IFor users working with different brands of confocal microscopes, this new version of bioImageL has implemented the function ‘Label Images,’ which can re-name images in stacks labeled by other software into the Nikon confocal software standards.
3D OPERATIONS
One of the main advantages of confocal laser microscopy is its ability to explore in situ biofilm sections without destruction of the specimens. To achieve this, 'stacks' of 2-D images, occupying the same x-y position but varying along the z-axis, are produced.
Stacks are used to reconstruct biofilm sections in 3-D and to further explore the volume and distribution of biofilms.
The function 'Surface and Volume Distribution' of bioImage_L allows individual stacks to be analyzed. Data obtained per stack of a biofilm population, as well as for its corresponding sub-populations (green and red), includes biovolume, mean height, and substratum coverage.
Additionally, this function reconstructs each individual subpopulation in the stack in 3-D.
Batch Cell Counting
Cell counting is also performed by bioImage_L for the analysis of multiple images stored in one folder.
In the function 'Cell Counting (Batch)', bioImage_L performs automatic cell counting for all images in a folder. All data obtained is stored in an Excel file.
2D OPERATIONS
The basic operative units in bioImage_L are performed in 2 dimensions. In these, cells are counted and distributed in sub-populations according to their fluorescent labeled color.
The basic operative units in bioImage_L are performed in 2 dimensions. In these, cells are counted and distributed in sub-populations according to their fluorescent labeled color.
With the functions 'Cell Counting' and 'Cell Counting (Batch)', analyses of individual and multiple images can be automatically accomplished.
bioImage_L Operations
