IFor users working with different brands of confocal microscopes, this new version of bioImageL has implemented the function ‘Label Images,’ which can re-name images in stacks labeled by other software into the Nikon confocal software standards.
3D OPERATIONS
​
One of the main advantages of confocal laser microscopy is its ability to explore in situ biofilm sections without destruction of the specimens. To achieve this, 'stacks' of 2-D images, occupying the same x-y position but varying along the z-axis, are produced.
​
Stacks are used to reconstruct biofilm sections in 3-D and to further explore the volume and distribution of biofilms.
The function 'Surface and Volume Distribution' of bioImage_L allows individual stacks to be analyzed. Data obtained per stack of a biofilm population, as well as for its corresponding sub-populations (green and red), includes biovolume, mean height, and substratum coverage.

Additionally, this function reconstructs each individual subpopulation in the stack in 3-D.
Batch Cell Counting
​
Cell counting is also performed by bioImage_L for the analysis of multiple images stored in one folder.
​
In the function 'Cell Counting (Batch)', bioImage_L performs automatic cell counting for all images in a folder. All data obtained is stored in an Excel file.
2D OPERATIONS
​
The basic operative units in bioImage_L are performed in 2 dimensions. In these, cells are counted and distributed in sub-populations according to their fluorescent labeled color.

The basic operative units in bioImage_L are performed in 2 dimensions. In these, cells are counted and distributed in sub-populations according to their fluorescent labeled color.
With the functions 'Cell Counting' and 'Cell Counting (Batch)', analyses of individual and multiple images can be automatically accomplished.
​
bioImage_L Operations
